Staphylococcus aureus

Usage

The workflow is designed for analyzing of Staphylococcus aureus.

Supported analysis

The workflow includes the following analysis in addition to species prediction and various methods for assessing the sequencing quality,

  • Antibiotic resistance prediction

  • Disinfectant resistance

  • Prediction of virulence factors

  • Plasmid detection

  • MLST

  • cgMLST

  • SCCmec type

  • spa type (for ONT workflow)

Software versions

Versions of softwares and containers used by the Staphylococcus aureus workflow.

Software

Version

Container

amrfinderplus

4.2.7

https://depot.galaxyproject.org/singularity/ncbi-amrfinderplus:4.2.7–hf69ffd2_0

bracken

2.8

https://depot.galaxyproject.org/singularity/bracken:2.8–py39hc16433a_0

bwa

0.7.17-r1188

https://depot.galaxyproject.org/singularity/bwakit:0.7.17.dev1–hdfd78af_1

chewbbaca

3.5.3

https://depot.galaxyproject.org/singularity/chewbbaca:3.5.3–pyh106432d_1

clair3

2.0.0

docker://hkubal/clair3:v2.0.0

fastqc

0.12.1

https://depot.galaxyproject.org/singularity/fastqc:0.12.1–hdfd78af_0

filtlong

0.3.1

https://depot.galaxyproject.org/singularity/filtlong:0.3.1–h077b44d_0

flye

2.9.6

https://depot.galaxyproject.org/singularity/flye:2.9.6–py39h475c85d_0

freebayes

1.3.6

https://depot.galaxyproject.org/singularity/freebayes:1.3.6–hbfe0e7f_2

hostile

2.0.0

https://depot.galaxyproject.org/singularity/hostile:2.0.0–pyhdfd78af_0

kraken2

2.17.1

https://depot.galaxyproject.org/singularity/mulled-v2-f8c4015c836dd3ce5c118cfed97ec8259bab9e9d:41404a18634d1ec6fe2a8a0dc54df6925d57fdbf-0

minimiap2

2.30

https://depot.galaxyproject.org/singularity/minimap2:2.30–h577a1d6_0

medaka

2.2.0

docker://ontresearch/medaka:shacf8338462607b17b1d68dbce212cb93daea50bad

mlst

2.23.0

https://depot.galaxyproject.org/singularity/mlst:2.23.0–hdfd78af_1

nanoplot

1.46.2

https://depot.galaxyproject.org/singularity/nanoplot:1.46.2–pyhdfd78af_0

plasmidfinder

2.1.6

https://depot.galaxyproject.org/singularity/plasmidfinder:2.1.6–py314hdfd78af_2

pointfinder_db

4.0.0

https://depot.galaxyproject.org/singularity/resfinder:4.4.2–pyhdfd78af_1

prp

1.6.1

docker://clinicalgenomicslund/bonsai-prp:1.6.1

quast

5.2.0

https://depot.galaxyproject.org/singularity/quast:5.2.0–py310pl5321hc8f18ef_2

resfinder

4.4.2

https://depot.galaxyproject.org/singularity/resfinder:4.4.2–pyhdfd78af_1

resfinder_db

2.1.1

https://depot.galaxyproject.org/singularity/resfinder:4.4.2–pyhdfd78af_1

samtools

1.17

https://depot.galaxyproject.org/singularity/samtools:1.17–h00cdaf9_0

sccmec

1.2.0

https://depot.galaxyproject.org/singularity/sccmec:1.2.0–hdfd78af_0

seqtk

1.4

https://depot.galaxyproject.org/singularity/seqtk:1.4–he4a0461_2

ska2

0.3.10

https://depot.galaxyproject.org/singularity/ska2:0.3.10–h4349ce8_0

skesa

2.5.1

https://depot.galaxyproject.org/singularity/skesa:2.5.1–hdcf5f25_0

sourmash

4.8.2

https://depot.galaxyproject.org/singularity/sourmash:4.8.2–hdfd78af_0

spades

3.15.5

https://depot.galaxyproject.org/singularity/spades:3.15.5–h95f258a_1

spatyper

0.3.3

https://depot.galaxyproject.org/singularity/spatyper:0.3.3–pyhdfd78af_3

trimmomatic

0.40

https://depot.galaxyproject.org/singularity/trimmomatic:0.40–hdfd78af_0

virulencefinder

2.0.4

https://depot.galaxyproject.org/singularity/virulencefinder:2.0.4–hdfd78af_1

virulencefinder_db

041b8b30ede055f92cbd8eaf3679ca7554857514

https://depot.galaxyproject.org/singularity/virulencefinder:2.0.4–hdfd78af_1